The Use of Agro-Industrial Waste Rich in Omega-3 PUFA during the Weaning Stress Improves the Gut Health of Weaned Piglets

(1) Background: The weaning period is a very important stage in the pig life, as during weaning, animals are susceptible to pathogens and develop postweaning diarrhoea. aim of our study was counteract stress improve piglets’ gut health by using nutritional intervention consisting mix agro-industrial wastes (grapeseed, flaxseed sea blackthorn meals) rich omega-3 PUFA. (2) Methods: Twelve cross-bred TOPIG hybrid piglets with an average body weight 11.25 kg were randomly distributed one two experimental groups: control group fed basic corn soybean diet (control diet) 10% mixture grapeseed, buckthorn meals ratio 3:4:1 (GFS diet). (3) Results: GFS had no effect on performance, biochemical parameters or total antibody synthesis. able significantly reduce concentration proinflammatory cytokines IL-1 beta TNF-alpha increases expression junction proteins (occludin, claudin 4, 7 extracellular protein matrix) at gene level compared control. presence increased abundance Bifidobacterium Lactobacillus species colonic content well propionic butyric acids. (4) Conclusions: Taken together, results showed that PUFA can be used ecological, environmentally friendly for improving negative effects associated stress.